We have reported the modification of acetaminophen polymer films with chitosan. In this paper, the electrochemical oxidation of acetaminophen in different pHs and conditions at the bare glassy carbon electrode. The bare glassy carbon electrode also exhibits a promising enhanced electrocatalytic activity towards the oxidation of acetaminophen. Different methods were used for the formation of poly-acetaminophen films and the deposition of chitosan. The presence of chitosan enhances the loaded and stability. Cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS) are used for the determination of acetaminophen and the apparent diffusion coefficient values for these compounds at different concentration as it gives some information about the kinetics of charge transfer during the redox reactions of these compounds. The effect of the method of poly-acetaminophen films formation on the synergism between the polymer film and the subsequently loaded chitosan is thoroughly studied. Finally, we have studied the surface morphology of the modified electrode using atomic force microscopy (AFM), which revealed that acetaminophen is coated on chitosan